Journal: Drug Design, Development and Therapy

Article Title: Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass

doi: 10.2147/DDDT.S281554

Figure Lengend Snippet: Adenovirus-encoding sh-PBEF reduced the increased phosphorylation of ERK1/2, Akt, and p38MAPK in rat lung tissue. ( A ) Representative blots; ( B ) Phosphorylation of ERK1/2; ( C ) Phosphorylation of AKT; ( D ) Phosphorylation of p38MAPK vs control; Original blots were shown in Supplemental figure 1.*P<0.05; vs LPS, # P<0.05; vs CPB+Blank, @ P<0.05.

Article Snippet: The membrane was incubated with the following antibodies overnight at 4°C: rabbit anti-PBEF (1:2000, 11776-1-AP, Proteintech); rabbit anti-ERK1/2 (1:1000, 16443-1-AP, Proteintech); rabbit anti-p-ERK1/2 (1:500, bs-3016R, Bioss); rabbit anti-p38MAPK (1:1000, bs-0637R, Bioss); rabbit anti-p-p38MAPK (1:1000, bs-5477R, Bioss); rabbit anti-AKT (1:500, ab8805, Abcam); rabbit anti-p-AKT (1:1000, bs-2720R, Bioss); rabbit anti-AQP1 (1:1000, bs-1506R, Bioss); rabbit anti-AQP5 (1:1000, AF5169, Affinity); rabbit anti-ENaC (1:1000, bs-2957R, Bioss); rabbit anti-surfactant protein D (SP) (1:1000, ab220422, Abcam); and mouse monoclonal anti-GAPDH (1:2000, TA-08, ZSBio).

Techniques:

Journal: Frontiers in pharmacology

Article Title: A Palmitoylethanolamide Producing Lactobacillus paracasei Improves Clostridium difficile Toxin A-Induced Colitis.

doi: 10.3389/fphar.2021.639728

Figure Lengend Snippet: FIGURE 4 | Prophylactic administration of pNAPE-LP and ultralow palmitate administration on pro-inflammatory signaling molecules expression and release in mice colon. Immunoreactive bands showing TLR-4, RhoA GTP, phosphorylated/unphosphorylated - p38 MAPK, NF-κB-related p50 and p65, and HIF-1α protein expression in both wild type (A) and PPARα KO (B) mice following TcdA challenge and their relative PPARα-dependent decrease following pNAPE-LP/palmitate co- administration. Relative densitometric analysis (C, D) of each protein (arbitrary units normalized on the expression of the housekeeping protein GAPDH). Results were expressed as mean ± SEM of n 6 experiments performed in triplicate. ***p < 0.001 vs. vehicle; and ooop < 0.001 and oop < 0.01 vs. TcdA. The figure also shows the effect of pNAPE-LP on TcdA challenged wild type (E) and PPARα KO mice (F) in terms of release of IL-6, nitric oxide (NO), and VEGF in the plasma. Results were expressed as mean ± SEM of n 6 experiments performed in triplicate. ***p < 0.001 vs. vehicle; and ooop < 0.001 vs. TcdA.

Article Snippet: After the transfer the membranes were incubated with 10% nonfat dry milk in PBS overnight at 4°C and then exposed, depending on the experiments, with rabbit polyclonal anti-NAPE-PLD (Abcam, Cambridge, United Kingdom) (1:200 v/v), rabbit polyclonal anti–toll-like receptor-4 (TLR4) (Bioss Antibodies, Boston, United States) (1:1,000 v/v), mouse monoclonal anti-RhoAGTPase (Santa Cruz Biotechnology, Santa Cruz, CA, United States) (1:100 v/v), rabbit polyclonal anti-p38 mitogen-activated protein kinase (p38 MAPK) (Bioss Antibodies, Boston, United States (1:1,000 v/v), rabbit monoclonal anti-phospho-p-38 (p-p38) MAPK (Santa Cruz Biotechnology, Santa Cruz, CA, United States) (1:1,000 v/v), rabbit polyclonal anti-NF-κB p65 (Sigma-Aldrich, Milan, Italy) (1:1,000 v/v), mouse monoclonal anti-NF-κB p50 (Santa Cruz Biotechnology, Santa Cruz, CA, United States) (1:1,000 v/v), mouse monoclonal anti–hypoxia-inducible factor-1-alpha (HIF-1α) (Novus biological, Abingdon, United Kingdom) (1: 500 v/v), and rabbit polyclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Danvers, MA, United States) (1:1,000 v/v) according to standard experimental protocols.

Techniques: Expressing, Clinical Proteomics

Journal: International immunopharmacology

Article Title: Hyperin attenuates inflammation by activating PPAR-γ in mice with acute liver injury (ALI) and LPS-induced RAW264.7 cells.

doi: 10.1016/j.intimp.2015.10.017

Figure Lengend Snippet: Fig. 4. Western blot analysis (A) showed that HP inhibited the expression of P-ERK1/2 and P-P38 protein in isolation of liver macrophages. HP inhibited the expression of P-ERK1/2 and P-P38 by Western blot and q-PCR (B) analysis in RAW264.7 cells. (All values are expressed as mean ± SEM. #P b 0.05, ##P b 0.01 compared with the control group, *P b 0.05, **P b 0.01 compared with the CCl4-induced group, LPS-induced group).

Article Snippet: Mouse monoclonal antibodies β-actin was purchased from Santa Cruz (California, USA) and rabbit ERK1/2, P-ERK1/2, P38, and P-P38 polyclonal antibodies were purchased from Bioss (Beijing, China).

Techniques: Western Blot, Expressing, Isolation, Control

Journal: International immunopharmacology

Article Title: Hyperin attenuates inflammation by activating PPAR-γ in mice with acute liver injury (ALI) and LPS-induced RAW264.7 cells.

doi: 10.1016/j.intimp.2015.10.017

Figure Lengend Snippet: Fig. 5. Western blot (A) analysis showed that the down-regulation of PPAR-γ obviously promoted P-ERK1/2 and P-P38 expression in RAW264.7 cells (All values are expressed as mean ± SEM. ##P b 0.01 compared with the control group, *P b 0.05, **P b 0.01 compared with LPS-induced group). While, Western blot (B) analysis showed P-ERK1/2 and P-P38 were activated remarkably after cells were treated with PPAR-γ si-RNA for 12 h. (#P b 0.05, ##P b 0.01, compared with LPS plus Nc group). The expression of P-ERK1/2 and P-P38 were obviously reduced because of the up-regulation of PPAR-γ by pEX-2-PPAR-γ(C). (#P b .0.05 compared with the pEX-2 group, *P b 0.05, **P b 0.05 compared with LPS plus pEX-2 group).

Article Snippet: Mouse monoclonal antibodies β-actin was purchased from Santa Cruz (California, USA) and rabbit ERK1/2, P-ERK1/2, P38, and P-P38 polyclonal antibodies were purchased from Bioss (Beijing, China).

Techniques: Western Blot, Expressing, Control

Journal: International immunopharmacology

Article Title: Hyperin attenuates inflammation by activating PPAR-γ in mice with acute liver injury (ALI) and LPS-induced RAW264.7 cells.

doi: 10.1016/j.intimp.2015.10.017

Figure Lengend Snippet: Fig. 6. HP activated target genes of PPAR-γ and up-regulated the expression of PPAR-γ in a dose-dependent manner in LPS-induced RAW264.7 cell. Then, it could inhibit the produc- tion of TNF-α and IL-6 through suppressing ERK1/2 and p38 MAPK activation.

Article Snippet: Mouse monoclonal antibodies β-actin was purchased from Santa Cruz (California, USA) and rabbit ERK1/2, P-ERK1/2, P38, and P-P38 polyclonal antibodies were purchased from Bioss (Beijing, China).

Techniques: Expressing, Activation Assay

Journal: Antioxidants

Article Title: Differences in Airway Remodeling and Emphysematous Lesions between Rats Exposed to Smoke from New-Type and Conventional Tobacco Varieties

doi: 10.3390/antiox13050511

Figure Lengend Snippet: Cigarette smoke-altered Nrf2 and p38 MAPK expression in the lung tissues of rats. Immunohistochemical staining of Nrf2 ( A – D ), p38 MAPK ( E – H ) and phospho-p38MAPK ( I – L ). Representative images at 60 days. 200×, scale bars = 50 µm. The relative expression levels of Nrf2 ( M ), p38 MAPK ( N ) and phosphor-p38 MAPK ( O ). The ratio of phosphor-p38 to p38 ( P ). Results are presented as means with standard deviations, as shown by the vertical bars (n = 6). The statistical significance between all data is compared. Bars with the same letter(s) indicate no significant differences at the level of p < 0.05.

Article Snippet: Rabbit polyclonal antibodies against Bcl-2 (26593-1-AP), Nrf2 (16396-1-AP), p38 MAPK (14064-1-AP) and phospho-p38 MAPK (Thr180/Tyr182) (28796-1-AP) were purchased from Proteintech (Wuhan, China).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SIRT1 protects against urban particulate matter-induced airway inflammation

doi: 10.2147/COPD.S202904

Figure Lengend Snippet: Resveratrol (Res) suppresses PM-induced inflammation response in HBE cells and mice via MAPK pathways. ( A ) HBE cells were treated with PM or resveratrol (Res), and the protein levels of MAPK pathways were measured by Western blot. ( B ) Mice were instilled intratracheally with normal saline (NS), PM or PM plus resveratrol respectively for 3 days, and the mice were sacrificed after 24 h. The protein levels of MAPK pathways were measured by Western blot. Data are representative of three independent studies. Data are representative of three independent studies. Results are expressed as mean ± SD. * p <0.05. Abbreviations: PM, Particulate matter; HBE, Human bronchial epithelial; MAPK, Mitogen-activated protein kinase.

Article Snippet: Antibodies against SIRT1 (Abcam, MA, USA, Catalog #110304), GAPDH (Sigma-Aldrich, Catalog #SAB2108266), p38 MAPK (CST, MA, USA, Catalog #8690), p-p38 MAPK (CST, MA, USA, Catalog #4511), p-ERK1/2 (CST, MA, USA, Catalog #4370), and ERK (CST, MA, USA, Catalog #4695), Resveratrol (Beyotime Biotechnology, Catalog #SC0276) were used.

Techniques: Western Blot, Saline

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SIRT1 protects against urban particulate matter-induced airway inflammation

doi: 10.2147/COPD.S202904

Figure Lengend Snippet: MAPK inhibitors suppress PM-induced inflammation response in HBE cells. ( A – I ) p38 inhibitor SB203580 and ERK inhibitor U0126 were added 2 h prior PM exposed and cells were collected after 24 h. The levels of TNF-α, IL-6 and, IL-17A were analyzed by RT-PCR. Data are representative of three independent studies. Results are expressed as mean ± SD. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001. Abbreviations: MAPK, Mitogen-activated protein kinase; PM, Particulate matter; HBE, Human bronchial epithelial; RT-PCR, Reverse Transcription-Polymerase Chain Reaction.

Article Snippet: Antibodies against SIRT1 (Abcam, MA, USA, Catalog #110304), GAPDH (Sigma-Aldrich, Catalog #SAB2108266), p38 MAPK (CST, MA, USA, Catalog #8690), p-p38 MAPK (CST, MA, USA, Catalog #4511), p-ERK1/2 (CST, MA, USA, Catalog #4370), and ERK (CST, MA, USA, Catalog #4695), Resveratrol (Beyotime Biotechnology, Catalog #SC0276) were used.

Techniques: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Polymerase Chain Reaction